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Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection

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dc.contributor.author Cacciotto, Carla
dc.contributor.author Addis, Maria Filippa
dc.contributor.author Coradduzza, Elisabetta
dc.contributor.author Carcangiu, Laura
dc.contributor.author Nuvoli, Anna Maria
dc.contributor.author Tore, Gessica
dc.contributor.author Dore, Gian Mario
dc.contributor.author Pagnozzi, Daniela
dc.contributor.author Uzzau, Sergio
dc.contributor.author Chessa, Bernardo
dc.contributor.author Pittau, Marco
dc.contributor.author Alberti, Alberto
dc.date.accessioned 2014-12-17T10:35:07Z
dc.date.available 2014-12-17T10:35:07Z
dc.date.issued 2013-02-28
dc.identifier.citation Cacciotto C, Addis MF, Coradduzza E, Carcangiu L, Nuvoli AM, et al. (2013) Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection. PLoS ONE 8(2): e57775 IT
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11050/1141
dc.description.abstract In this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed in Escherichia coli as a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2 at temperatures ranging from 37 to 45°C. According to in silico analyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to the Mycoplasma hominis group. In M. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role in Mycoplasma agalactiae survival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants. IT
dc.language.iso en IT
dc.publisher Public Library of Science IT
dc.relation.ispartof PLoS ONE IT
dc.relation.ispartofseries 8;2
dc.rights Attribuzione - Non commerciale - Non opere derivate 3.0 Italia *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/it/ *
dc.subject Mycoplasma agalactiae IT
dc.subject sheep IT
dc.subject recombinant proteins IT
dc.subject MAG_5040 IT
dc.subject Escherichia coli IT
dc.subject nucleic acids IT
dc.title Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection IT
dc.type Articolo IT
dc.description.status Pubblicato IT
dc.identifier.doi 10.1371/journal.pone.0057775 IT
dc.subject.een-cordis EEN CORDIS::SCIENZE BIOLOGICHE ::Biologia / biotecnologia ::Microbiologia IT
dc.subject.een-cordis EEN CORDIS::AGRICOLTURA E RISORSE MARINE::Agricoltura::Produzione animale / allevamento IT


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Attribuzione - Non commerciale - Non opere derivate 3.0 Italia Attribuzione - Non commerciale - Non opere derivate 3.0 Italia