P-arch - Digital Repository

An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid

Mostra i principali dati

dc.contributor.author Pagnozzi, Daniela
dc.contributor.author Biosa, Grazia
dc.contributor.author Addis, Maria Filippa
dc.contributor.author Mastrandrea, Scilla
dc.contributor.author Masala, Giovanna
dc.contributor.author Uzzau, Sergio
dc.date.accessioned 2014-12-09T12:00:44Z
dc.date.available 2014-12-09T12:00:44Z
dc.date.issued 2014-08-13
dc.identifier.citation Pagnozzi D, Biosa G, Addis MF, Mastrandrea S, Masala G, et al. (2014) An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid. PLoS ONE 9(8): e104962. doi:10.1371/journal.pone.0104962 IT
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11050/1132
dc.description.abstract Background: Currently, the serodiagnosis of cystic echinococcosis relies mostly on crude Echinococcus granulosus hydatid cyst fluid as the antigen. Consequently, available immunodiagnostic tests lack standardization of the target antigen and, in turn, this is reflected on poor sensitivity and specificity of the serological diagnosis. Methodology/Principal Findings: Here, a chromatographic method enabling the generation of highly enriched Antigen 5 (Ag5) is described. The procedure is very easy, efficient and reproducible, since different hydatid cyst fluid (HCF) sources produced very similar chromatograms, notwithstanding the clearly evident and extreme heterogeneity of the starting material. In addition, the performance of the antigen preparation in immunological assays was preliminarily assessed by western immunoblotting and ELISA on a limited panel of cystic echinococcosis patients and healthy controls. Following western immunoblotting and ELISA experiments, a high reactivity of patient sera was seen, with unambiguous and highly specific results. Conclusions/Significance: The methods and results reported open interesting perspectives for the development of sensitive diagnostic tools to enable the timely and unambiguous detection of cystic echinococcosis antibodies in patient sera. IT
dc.description.sponsorship This work was supported by Regione Autonoma della Sardegna (http://www.regione.sardegna.it/) IT
dc.language.iso en IT
dc.publisher Public Library of Science IT
dc.relation.ispartof PLoS ONE IT
dc.relation.ispartofseries 9;8
dc.rights Attribuzione - Non commerciale - Non opere derivate 3.0 Italia *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/it/ *
dc.subject antigens IT
dc.subject diagnostic medicine IT
dc.subject echinococcosis IT
dc.subject enzyme-linked immunoassays IT
dc.subject serum proteins IT
dc.subject membrane proteins IT
dc.subject sheep IT
dc.subject size-exclusion chromatography IT
dc.title An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid IT
dc.type Articolo IT
dc.description.status Pubblicato IT
dc.identifier.doi 10.1371/journal.pone.0104962 IT
dc.subject.een-cordis EEN CORDIS::SCIENZE BIOLOGICHE ::Medicina, salute umana::Apparecchiature medicali ed elettromedicali IT
dc.subject.een-cordis EEN CORDIS::SCIENZE BIOLOGICHE ::Ricerca sul genoma ::Espressione genica, ricerca proteomica IT
dc.identifier.eissn 1932-6203


File allegati

I seguenti file di Licenza sono associati a questo inserimento:

Questo inserimento fa parte delle seguenti collezioni

Mostra i principali dati

Attribuzione - Non commerciale - Non opere derivate 3.0 Italia Attribuzione - Non commerciale - Non opere derivate 3.0 Italia